SNFGE SNFGE
 
Thématique :
- Foie
Originalité :
Réexamen
Solidité :
Intermédiaire
Doit faire évoluer notre pratique :
Pas encore
 
 
Nom du veilleur :
Professeur Christine SILVAIN
Coup de coeur :
 
 
Hepatology
  2016/12  
 
  2016 Dec;64(6):2015-2027  
  doi: 10.1002/hep.28829.  
 
  Changes in blood microbiota profiles associated with liver fibrosis in obese patients: A pilot analysis  
 
  Lelouvier B, Servant F, Païssé S, Brunet AC, Benyahya S, Serino M, Valle C, Ortiz MR, Puig J, Courtney M, Federici M, Fernández-Real JM, Burcelin R, Amar  
  https://www.ncbi.nlm.nih.gov/pubmed/27639192  
 
 

Abstract

The early detection of liver fibrosis among patients with nonalcoholic fatty liver disease (NAFLD) is an important clinical need. In view of the suggested role played by bacterial translocation in liver disease and obesity, we sought to investigate the relationship between blood microbiota and liver fibrosis (LF) in European cohorts of patients with severe obesity. We carried out a cross-sectional study of obese patients, well characterized with respect to the severity of the NAFLD, in the cohort FLORINASH. This cohort has been divided into a discovery cohort comprising 50 Spanish patients and then in a validation cohort of 71 Italian patients. Blood bacterial DNA was analyzed both quantitatively by 16S ribosomal DNA (rDNA) quantitative polymerase chain reaction and qualitatively by 16S rDNA targeted metagenomic sequencing and functional metagenome prediction. Spanish plasma bile acid contents were analyzed by liquid chromatography/mass spectrometry. The 16S rDNA concentration was significantly higher in patients of the discovery cohort with LF. By 16S sequencing, we found specific differences in the proportion of several bacterial taxa in both blood and feces that correlate with the presence of LF, thus defining a specific signature of the liver disease. Several secondary/primary bile acid ratios were also decreased with LF in the discovery cohort. We confirmed, in the validation cohort, the correlation between blood 16S rDNA concentration and LF, whereas we did not confirm the specific bacterial taxa signature, despite a similar trend in patients with more-severe fibrosis.

CONCLUSION:

Changes in blood microbiota are associated with LF in obese patients. Blood microbiota analysis provides potential biomarkers for the detection of LF in this population.

 

 
Question posée
 
Relations entre le microbiote sanguin et la fibrose hépatique dans de cohorte de patients avec obésité sévère (FLORINASH) et bien caractérisés quant à la sévérité de la NAFLD.
 
Question posée
 
La concentration en 16S ADNr est significativement plus élevée dans la première cohorte avec fibrose hépatique et cette corrélation est retrouvée dans la cohorte de validation.
 
Commentaires

Des données complémentaires confirmant que l’analyse du microbiote sanguin serait un biomarqueur indirect de la fibrose hépatique chez les patients obèses : à corréler avec d’autres marqueurs indirects.

 
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