Thématique :
- Colo-proctologie
Originalité :
Solidité :
Doit faire évoluer notre pratique :
Dans certains cas
Nom du veilleur :
Docteur Pauline JOUET
Coup de coeur :
  2017 Sep;153(3):689-701.e1.  
  doi: 10.1053/j.gastro.2017.05.015.  
  Tests for Serum Transglutaminase and Endomysial Antibodies Do Not Detect Most Patients With Celiac Disease and Persistent Villous Atrophy on Gluten-free Diets: a Meta-analysis.  
  Silvester JA, Kurada S, Szwajcer A, Kelly CP, Leffler DA, Duerksen DR  



Tests to measure serum endomysial antibodies (EMA) and antibodies to tissue transglutaminase (tTG) were developed to screen for celiac disease in patients consuming gluten. However, they are commonly used to monitor patients on a gluten-free diet (GFD). We conducted a meta-analysis to assess the sensitivity and specificity of tTG IgA and EMA IgA assays in identifying patients with celiac disease who have persistent villous atrophy despite a GFD.


We searched PUBMED, EMBASE, BIOSIS, SCOPUS,, Science Citation Index, and Cochrane Library databases through November 2016. Inclusion criteria were studies of subjects with biopsy-confirmed celiac disease, follow-up biopsies, and measurement of serum antibodies on a GFD, biopsy performed on subjects regardless of symptoms, or antibody test results. Our analysis excluded subjects with refractory celiac disease, undergoing gluten challenge, or consuming a prescribed oats-containing GFD. Tests were considered to have positive or negative findings based on manufacturer cut-off values. Villous atrophy was defined as a Marsh 3 lesion or villous height:crypt depth ratio below 3.0. We constructed forest plots to determine the sensitivity and specificity of detection for individual studies. For the meta-analysis, a bivariate random effects model was used to jointly model sensitivity and specificity.


Our search identified 5408 unique citations. Following review of abstracts, 442 articles were reviewed in detail. Only 26 studies (6 of tTG assays, 15 of EMA assays, and 5 of tTG and EMA assays) met our inclusion criteria. The most common reason studies were excluded from our analysis was inability to cross-tabulate histologic and serologic findings. The serum assays identified patients with persistent villous atrophy with high levels of specificity: 0.83 for the tTG IgA assay (95% CI, 0.79-0.87) and 0.91 for the EMA IgA assay (95% CI, 0.87-0.94). However, they detected villous atrophy with low levels of sensitivity: 0.50 for the tTG IgA assay (95% CI, 0.41-0.60) and 0.45 for the EMA IgA assay (95% CI, 0.34-0.57). The tests had similar levels of performance in pediatric and adult patients.


In a meta-analysis of patients with biopsy-confirmed celiac disease undergoing follow-up biopsy on a GFD, we found that tests for serum tTG IgA and EMA IgA levels had low sensitivity (below 50%) in detection of persistent villous atrophy. We need more-accurate non-invasive markers of mucosal damage in children and adults with celiac disease who are following a GFD.


Question posée
Quelle est la sensibilité et la spécificité des Ig A anti-transglutaminase et anti-endomysium pour identifier les patients avec maladie coeliaque ayant une atrophie villositaire persistante sous régime sans gluten ?
Question posée
Dans cette méta-analyse effectuée chez des patients ayant une maladie coeliaque histologiquement prouvée, la sensibilité de ces tests était faible aussi bien chez l’adulte que chez l’enfant, de 0,5 (IC 95% 0,41-0,60) pour les anti-transglutaminases et de 0,45 (IC 95% 0,34-0,57) pour les anti-endomysium. Par contre la spécificité était élevée : 0,83 (IC 95% 0,79-0,87) et 0,91 (IC 95% 0,87-0,94) respectivement.

La négativité des IgA anti-transglutaminases et anti-endomysium ne suffit pas pour confirmer la disparition de l’atrophie villositaire.